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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become observed in many tumors, which ends up in chromosome instability, and thus might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial function in selling cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually explained. Two extra splice variants were being documented but comprehensive nucleotide sequence facts is not nevertheless accessible.

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Blocking peptides are peptides that bind particularly on the target antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.

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This guide outlines even more achievable optimization of cellular and immunoassay parameters to ensure the absolute best outcomes are...

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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make sure the absolute best final results are...

Antibodies bound to the blocking peptide no longer bind towards the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.

Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic function. Cyclin E sorts a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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